Genomics-assisted exploitation of barley diversity (EXBARDIV):
This project, running from 2007-2011, was a collaboration between Andy Flavell (Coordinator; University of Dundee at SCRI), Klaus Pillen (Max Planck Institute, Köln), Alan Schulman (MTT Helsinki), Andreas Graner (IPK-Gatersleben), Luigi Cattivelli (CRA-IECR, Fiorenzuola d’Arda), Søren Rasmussen (Faculty Life Sciences, University of Copenhagen) and Joanne Russell (Scottish Crop Research Institute, Invergowrie)
Funding: 2.4 million Euros
The central goal of this project is to establish an incremental association mapping approach based on different population types for the discovery of new gene alleles in wild and landrace barley, which can be exploited for crop breeding. Our approach will build upon the strong genomics base of barley and will apply association genetics concepts pioneered in humans and Arabidopsis to test the efficiency of the association genetics approach for identifying gene alleles in Hordeum that are needed by the breeder. Our second objective is to recruit the new useful gene alleles, which have been discovered in the above studies, into advanced back-cross (ABC) breeding programs derived from wide crosses between H. spontaneum germplasm and elite cultivars. This will allow us to determine the efficiencies of identification and extraction of useful alleles in barley breeding programs based upon wide crosses. Our third major project objective is to use the huge DNA and marker data set obtained in the project to determine important population genetic parameters for barley.
1. Moragues, M, Comadran, J, Waugh, R, Milne, I, Flavell, AJ & Russell, JR. (2010) Effects of ascertainment bias and marker number on estimations of barley diversity from high throughput SNP genotype data Theor. Appl. Genet. 120:1525-1534
2. Russell J, Dawson IK, Flavell AJ, Steffenson B, Weltzien E, Booth A, Ceccarelli S, Grando S & Waugh R (2011) Analysis of more than 1,000 SNPs in geographically-matched samples of landrace and wildbarley indicates secondary contact and chromosome-level differences in diversity around domestication genes. New Phytologist 191:564-578.
3. Newton AC, Flavell AJ, George TS, Leat P, Mulholland B, Ramsay, L, Revoredo-Giha C, Russell J, Steffenson B, Swanston S, Thomas WTB, Waugh R, White PJ and Bingham IJ (2011) Crops the feed the World 4. Barley: a resilient crop? Strengths and weaknesses in the face of food security. Food Security 3:141-178.
4. Hübner S, Günther T, Flavell AJ, Fridman E, Graner A, Korol A and Schmid KJ (2012) Islands and Streams: Clusters and Gene Flow in Wild Barley Populations From the Levant. Molecular Ecology 21: 1115-1129.
5. Tondelli A, Xin X, Moragues M, Sharma R,Schnaithmann F, Ingvardsen C, Manninen O, Comadran J, Russell J, Waugh R, Schulman AH, Pillen K, Rasmussen SK, Kilian B, Cattivelli L, Thomas WTB and Flavell AJ (2013) Structural and temporal variation in genetic diversity of European spring 2-row barleycultivars and association mapping of quantitative traits. The Plant Genome 6: doi:10.3835/plantgenome2013.03.0007.
6. Tondelli A, Pagani D, Ghafoori IN, Ataei MRR, Rizza F, Flavell AJ, Cattivelli L (2014) Allelic variation at Fr-H1/Vrn-H1 and Fr-H2 loci is the main determinant of frost tolerance in spring barley. Environmental and Experimental Botany doi: 10.1016/j.envexpbot.2014.02.014.
For further information on this project please contact Andy Flavell (email@example.com) from the James Hutton Institute / University of Dundee.